Improvement of the indirect hemagglutination assay for Salmonella typhi Vi antibodies by use of glutaraldehyde-fixed erythrocytes.
نویسنده
چکیده
Glutaraldehyde-fixed sheep erythrocytes sensitized with Vi antigen were shown to be usable for at least 1 year in the indirect hemagglutination assay for Vi antibodies. The use of fixed sensitized erythrocytes improves the practicality of the indirect hemagglutination assay by (i) eliminating the need to sensitize cells each time the test is performed; (ii) reducing waste of the purified Vi antigen; and (iii) reducing test-to-test variation.
منابع مشابه
Development and evaluation of an enzyme-linked immunosorbent assay for serum Vi antibodies for detection of chronic Salmonella typhi carriers.
An enzyme-linked immunosorbent assay (ELISA) measuring, in serum, immunoglobulin G (IgG), IgM, and IgA to Vi capsular polysaccharide antigen that was tyraminated (Vi-Tyr) to increase its binding efficiency to microtiter plates was compared with the standard passive hemagglutination assay (PHA) as a screening test for chronic Salmonella typhi carriers. Initially, three populations were evaluated...
متن کاملPreparation of stable sensitized erythrocytes for detection of chlamydial antibodies.
Sheep erythrocytes were treated with glutaraldehyde before sensitization for the indirect hemagglutination test to assay chlamydial antibodies. This treatment markedly increased stability during storage.
متن کاملEvaluation of modified passive haemagglutination assay for Vi antibody estimation in Salmonella typhi infections.
A simple passive haemmagglutination assay (PHA) was developed to detect Vi antibodies, to improve the diagnosis of typhoid fever by small laboratories. The Vi capsular antigen of Salmonella typhi was extracted by alternate alcohol and acetone precipitation. Formalin fixed, sheep red blood cells treated with chromium chloride were sensitised with this Vi antigen and antibodies detected and measu...
متن کاملEvaluation of a new assay for Vi antibody in chronic carriers of Salmonella typhi.
The assay for serum antibody to the Salmonella typhi capsular polysaccharide (Vi) antigen has recently been revised because of the availability of a purified, highly polymerized Vi antigen. We compared this revised Vi antibody assay to the traditional one for potential usefulness in the surveillance of chronic enteric carriers of S. typhi. The purified Vi antigen of Citrobacter freundii was inc...
متن کاملUse of glutaraldehyde-fixed goose erythrocytes in arbovirus serology.
Goose erythrocytes preserved with glutaraldehyde were compared with fresh cells in hemagglutination and hemagglutination-inhibition tests for arbovirus antigens and antibodies. The glutaraldehyde-fixed cells were as sensitive and specific as theresh erythrocytes and were stable at 4 degrees C for 6 months.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 22 4 شماره
صفحات -
تاریخ انتشار 1985